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Samanta, Priyanka
- Isolation and Characterization of Amylase Producing Fungal Strain
Abstract Views :241 |
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Authors
Affiliations
1 Department of Biotechnology, School of Biotechnology & Biosciences, Brainware University, 398, Ramkrishnapur Road, Barasat, Kolkata 700125, IN
1 Department of Biotechnology, School of Biotechnology & Biosciences, Brainware University, 398, Ramkrishnapur Road, Barasat, Kolkata 700125, IN
Source
Journal of Environment and Sociobiology, Vol 17, No 2 (2020), Pagination: 1-7Abstract
Research on Amylase has significantly increased due to its potential applications in paper and pulp industries, enzymatic scarification and liquefaction of starch, textile processes, baking industry and detergent industries. The present study was aimed to isolate the potent amylase producing fungal strain. Five different fungal strains were isolated from the garden soil of Brainware University, Kolkata, India. After screening, three out of these strains were found to have amylolytic activity. These three strains were further screened for production of amylase in liquid medium.Keywords
Amylase production, Fungi, Strain isolation, Fermentation.- Analysis of Relative DNA Content of Termitomyces Protoplast using Propidium Iodide
Abstract Views :253 |
PDF Views:1
Authors
Affiliations
1 Department of Biotechnology, School of Biotechnology & Biosciences, Brainware University, 398, Ramkrishnapur Road, Barasat, North 24 Parganas, Kolkata-700125, IN
2 Division of Plant Biology, Bose Institute, P-1/12, CIT Rd, Scheme VIIM, Kankurgachi, Kolkata-700054, IN
1 Department of Biotechnology, School of Biotechnology & Biosciences, Brainware University, 398, Ramkrishnapur Road, Barasat, North 24 Parganas, Kolkata-700125, IN
2 Division of Plant Biology, Bose Institute, P-1/12, CIT Rd, Scheme VIIM, Kankurgachi, Kolkata-700054, IN
Source
Journal of Environment and Sociobiology, Vol 17, No 2 (2020), Pagination: 15-18Abstract
Towards establishment of biodiversity character via analysis of relative DNA content of wild edible mushroom, Termitomyces species is used here for the first time. Dye propidium iodide (PI), an intercalating fluorescent molecule with molecular mass of 668.4 Da is used for staining of Termitomyces protoplasts to analyse the cellular DNA content using flow cytometer. PI binds the cellular nucleic acids at 535 nm excitation and 617 nm emission range. The protoplasts were isolated from liquid MYG grown vegetative tissues of edible mushroom strain Termitomyces heimii. The isolated protoplasts were purified with 0.6M manitol (MW 3350) followed by washing in PBS buffer. Protoplast pellet was suspended in PI (l mg/ml) hypotonic solution and final volume made up with sterile water. Incubation was done at 37°C for 30 min followed by analyzing using flow cytometer. Results showed that staining depends upon the protoplast purity, size and viability. The vegetative tissue showed a heterogeneous protoplast population in terms of sizes which is ultimately reflected on the relative amount of DNA content. The heterogeneous protoplast population in the suspension through cytometry score results the unequal amount of DNA content in every cell meaning unequal size, age and viability. It is established that PI can be used for staining of mushroom protoplast in biodiversity and genetic research.Keywords
Edible mushroom, Protoplast, Propidium iodide, Flow cytometry, DNA content.- Bio-inspired Synthesis of Silver Nanoparticles and their Potential Antibacterial Applications
Abstract Views :243 |
PDF Views:1
Authors
Affiliations
1 Department of Biotechnology, Brainware University, Barasat, Kolkata, 700125, West Bengal, IN
1 Department of Biotechnology, Brainware University, Barasat, Kolkata, 700125, West Bengal, IN
Source
Journal of Environment and Sociobiology, Vol 17, No 2 (2020), Pagination: 19-25Abstract
The use of phytochemicals for the synthesis of silver nanoparticles (AgNPs) is economical, easily accessible and harmless, and that can provide nanoparticles of controlled size and morphology. The current study focused on the green, quick, simplistic and cost-effective synthesis of AgNPs using Vaccinium corymbosum, a perennial flowering plant with blue or purple-colored berries, and antibacterial potential of synthesized AgNPs. UV-Visible and Fourier transform infrared spectroscopy analyses confirmed the formation of AgNPs. DLS analysis showed that the phytochemicals present in blue berries resulted in monodispersed spherical AgNPs in the size range of 40 to 80 nm. AgNPs possessed potential antibacterial activity against Escherichia coli and Staphylococcus aureus, and were nontoxic. Therefore, green synthesis of nano silvers has the potential to serve as a powerful antimicrobial agent for various therapeutic applications.Keywords
Silver Nanoparticle, Phytochemicals, Antibacterial, Blue Berries.- Study on Silver Nanoparticles Induced Shoot Growth of a Medicinal Plant
Abstract Views :247 |
PDF Views:1
Authors
Affiliations
1 Department of Biotechnology, Brainware University, Kolkata-700125, IN
1 Department of Biotechnology, Brainware University, Kolkata-700125, IN
Source
Journal of Environment and Sociobiology, Vol 17, No 2 (2020), Pagination: 27-30Abstract
Random extraction of wild stock of a medicinalplant, Swertia chiratacaused marked depletion of natural population of the plant. In vitro tissue propagation helps in maintaining stock and availability of the plant in need. Application of nanoparticles encapped byphytochemicals on regenerating shoot meristems enhance shoot proliferation. Maintenance of Reactive oxygen species (ROS) balance and better bio-acceptance of silver nanoparticles play vital role in improved in shoot regeneration and proliferation of the plant. The outcome of the study may help conservation of medicinal plant species.Keywords
Silver Nanoparticles Plant Tissue Culture · Shoot Regeneration. Reactive Oxygen Species.- Antimicrobial Efficacy of OcimumM Plants
Abstract Views :226 |
PDF Views:1
Authors
Affiliations
1 Department of Biotechnology, Brainware University, Kolkata-700125, IN
2 Department of Rog Nidan (Pathology), Institute of Post Graduate Ayurvedic Education & Research, SVSP Hospital, Kolkata-700009, IN
1 Department of Biotechnology, Brainware University, Kolkata-700125, IN
2 Department of Rog Nidan (Pathology), Institute of Post Graduate Ayurvedic Education & Research, SVSP Hospital, Kolkata-700009, IN